Researchers used a noninvasive and label-free method called optical coherence tomography to assess the live cells in the 3D scaffold system. OCT is an imaging technique that uses light waves to take cross sectional images of a sample.
Credit: R. Wilson/NIST
Engineering organs to replace damaged hearts or kidneys in the human body may seem like something out of a sci-fi movie, but the building blocks for this technology are already in place. In the burgeoning field of tissue engineering, live cells grow in artificial scaffolds to form biological tissue. But to evaluate how successfully the cells develop into tissue, researchers need a reliable method to monitor the cells as they move and multiply.
Now, scientists at the National Institute of Standards and Technology (NIST), the U.S Food and Drug Administration (FDA) and the National Institutes of Health (NIH) have developed a noninvasive method to count the live cells in a three-dimensional (3D) scaffold. The real-time technique images millimeter-scale regions to assess the viability of the cells and how the cells are distributed within the scaffold — an important capability for researchers who manufacture complex biological tissues from simple materials such as living cells.
Their findings have been published in the Journal of Biomedical Materials Research Part A.
First, researchers created a 3D scaffold system made from a network of polymer molecules that can hold large amounts of water, forming a type of material known as a hydrogel. The 3D hydrogel was then embedded with a type of human white blood cell that can reproduce endl ..
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